Separation and Identification of Permethylated Glycan Isomers by Reversed Phase NanoLC-NSI-MSⁿ.

ABSTRACT: HPLC has been employed for decades to enhance detection sensitivity and quantification of complex analytes within biological mixtures. Among these analytes, glycans released from glycoproteins and glycolipids have been characterized as underivatized or fluorescently tagged derivatives by HPLC coupled to various detection methods. These approaches have proven extremely useful for profiling the structural diversity of glycoprotein and glycolipid glycosylation but require the availability of glycan standards and secondary orthogonal degradation strategies to validate structural assignments. A robust method for HPLC separation of glycans as their permethylated derivatives, coupled with in-line multidimensional ion fragmentation (MSⁿ) to assign structural features independent of stan

SUBMITTER: Kurz S 

PROVIDER: S-EPMC8724860 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature