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Crystal structures of FolM alternative dihydrofolate reductase 1 from Brucella suis and Brucella canis.

ABSTRACT: Members of the bacterial genus Brucella cause brucellosis, a zoonotic disease that affects both livestock and wildlife. Brucella are category B infectious agents that can be aerosolized for biological warfare. As part of the structural genomics studies at the Seattle Structural Genomics Center for Infectious Disease (SSGCID), FolM alternative dihydrofolate reductases 1 from Brucella suis and Brucella canis were produced and their structures are reported. The enzymes share ∼95% sequence identity but have less than 33% sequence identity to other homologues with known structure. The structures are prototypical NADPH-dependent short-chain reductases that share their highest tertiary-structural similarity with protozoan pteridine reductases, which are being investigated for rational therapeutic development.

SUBMITTER: Porter I 

PROVIDER: S-EPMC8725004 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature

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Crystal structures of FolM alternative dihydrofolate reductase 1 from Brucella suis and Brucella canis.

Porter Imani I   Neal Trinity T   Walker Zion Z   Hayes Dylan D   Fowler Kayla K   Billups Nyah N   Rhoades Anais A   Smith Christian C   Smith Kaelyn K   Staker Bart L BL   Dranow David M DM   Mayclin Stephen J SJ   Subramanian Sandhya S   Edwards Thomas E TE   Myler Peter J PJ   Asojo Oluwatoyin A OA  

Acta crystallographica. Section F, Structural biology communications 20220101 Pt 1

Members of the bacterial genus Brucella cause brucellosis, a zoonotic disease that affects both livestock and wildlife. Brucella are category B infectious agents that can be aerosolized for biological warfare. As part of the structural genomics studies at the Seattle Structural Genomics Center for Infectious Disease (SSGCID), FolM alternative dihydrofolate reductases 1 from Brucella suis and Brucella canis were produced and their structures are reported. The enzymes share ∼95% sequence identity  ...[more]

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