Splenic CD4⁺ and CD8⁺ T-cells highly expressed PD-1 and Tim-3 in cirrhotic patients with HCV infection and portal hypertension.

ABSTRACT: Introduction: The spleen plays an important role in regulating the immune response to infectious pathogens. T-cells dysfunction and exhaustion have been reported in patients with hepatitis B/C virus (HBV/HCV) infection, which contributes to persistent virus infection. The aims of this study were to investigate spleen-related evidence of immunosuppression and immune tolerance in HCV cirrhotic patients with portal hypertension (PH). Methods: The expression of programmed cell death 1 (PD-1), T-cell immunoglobulin domain and mucin domain-containing molecule-3 (Tim-3) and its ligand PD-L1/2, and Galectin-9 in the spleens and livers of HCV cirrhotic patients (n = 15) was analyzed using real-time PCR and immunohistochemistry. Flow cytometry was used to evaluate the expression

SUBMITTER: Huang N 

PROVIDER: S-EPMC8725229 | biostudies-literature | 2021 Jan-Dec

REPOSITORIES: biostudies-literature