TMT-based quantitative proteomic profiling of human monocyte-derived macrophages and foam cells.

ABSTRACT:

Background

Cardiovascular diseases remain the leading cause of morbidity and mortality worldwide, most of which are caused by atherosclerosis. Discerning processes that participate in macrophage-to-foam cell formation are critical for understanding the basic mechanisms underlying atherosclerosis. To explore the molecular mechanisms of foam cell formation, differentially expressed proteins were identified.

Methods

Human peripheral blood mononuclear cells were stimulated with macrophage colony-stimulating factor, and obtained macrophages were transformed into foam cells by oxidized low-density lipoprotein. Tandem mass tag (TMT) labeling combined with mass spectrometry was performed to find associations between foam cell transformation and proteome profiles.

Results

Tota

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC8725474 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature