TSH stimulation of human thyroglobulin and thyroid peroxidase gene transcription is partially dependent on internalization.

ABSTRACT: The TSH receptor (TSHR) is the major regulator of thyroid hormone biosynthesis in human thyrocytes by regulating the transcription of a number of genes including thyroglobulin (TG) and thyroperoxidase (TPO). Until recently, it was thought that TSHR initiated signal transduction pathways only at the cell-surface and that internalization was primarily involved in TSHR desensitization and downregulation. Studies primarily in mouse cells showed that TSHR internalization regulates gene transcription at an intracellular site also. However, this has not been shown for genes involved in thyroid hormone biosynthesis in human thyrocytes. We used human thyrocytes in primary culture. In these cells, the dose-response to TSH for gene expression is biphasic with low doses upregulating gene expression an

SUBMITTER: Jang D 

PROVIDER: S-EPMC8725617 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature