Development of antibacterial composite resin containing chitosan/fluoride microparticles as pit and fissure sealant to prevent caries.

ABSTRACT:

Objective

Develop a fissure sealant containing chitosan/fluoride microparticles (C/F) with antibacterial, fluoride release and recharge ability.

Materials and methods

Chitosan/fluoride microparticles were synthesized and added to Bis-GMA as C/F. The experimental group comprised 0%, 2%, 4% C/F, with Clinpro^TM fissure sealant as control. Antibacterial activity was detected by Alamar Blue assay and colony-forming units (CFU). Biocompatibility was determined by WST-1 and LDH test. Curing depth, flowability, tensile strength and flexural strength were measured according to the ISO standard; microhardness by Vickers hardness test. Fluoride release and recharge were recorded through ionic chromatography. Statistical analysis was performed with an independent t-test, one-

SUBMITTER: Lai CC 

PROVIDER: S-EPMC8725701 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature