Fully automated expansion and activation of clinical-grade natural killer cells for adoptive immunotherapy.

ABSTRACT:

Background aims

Ex vivo expansion of natural killer (NK) cells is a strategy to produce large numbers of these effector cells for immunotherapy. However, the transfer of bench-top expansion protocols to clinically applicable methods is challenging for NK cell-based therapy because of regulatory aspects and scale-up issues. Therefore, we developed an automated, large-scale NK cell expansion process.

Methods

Enriched NK cells were expanded with interleukin-2 and irradiated clinical-grade Epstein-Barr virus-transformed lymphoblastoid feeder cells with the use of an automated system in comparison to manual expansion, and the cells were investigated for their functionality, phenotype and gene expression.

Results

Automated expansion resulted in a mean 850-fold expansion of

SUBMITTER: Granzin M 

PROVIDER: S-EPMC8725994 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature