An integrated workflow for enhanced taxonomic and functional coverage of the mouse fecal metaproteome.

ABSTRACT: Intestinal microbiota plays a key role in shaping host homeostasis by regulating metabolism, immune responses and behavior. Its dysregulation has been associated with metabolic, immune and neuropsychiatric disorders and is accompanied by changes in bacterial metabolic regulation. Although proteomics is well suited for analysis of individual microbes, metaproteomics of fecal samples is challenging due to the physical structure of the sample, presence of contaminating host proteins and coexistence of hundreds of taxa. Furthermore, there is a lack of consensus regarding preparation of fecal samples, as well as downstream bioinformatic analyses following metaproteomics data acquisition. Here we assess sample preparation and data analysis strategies applied to mouse feces in a typical mass spec

SUBMITTER: Nalpas N 

PROVIDER: S-EPMC8726736 | biostudies-literature | 2021 Jan-Dec

REPOSITORIES: biostudies-literature