Effect of duty cycles of tumor‑treating fields on glioblastoma cells and normal brain organoids.

ABSTRACT: Tumor‑treating fields (TTFields) are emerging cancer therapies based on alternating low‑intensity electric fields that interfere with dividing cells and induce cancer cell apoptosis. However, to date, there is limited knowledge of their effects on normal cells, as well as the effects of different duty cycles on outcomes. The present study evaluated the effects of TTFields with different duty cycles on glioma spheroid cells and normal brain organoids. A customized TTFields system was developed to perform in vitro experiments with varying duty cycles. Three duty cycles were applied to three types of glioma spheroid cells and brain organoids. The efficacy and safety of the TTFields were evaluated by analyzing the cell cycle of glioma cells, and markers of neural stem cells (NSCs) and a

SUBMITTER: Ye E 

PROVIDER: S-EPMC8727135 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature