A stem cell marker KLF5 regulates CCAT1 via three-dimensional genome structure in colorectal cancer cells.

ABSTRACT:

Background

KLF5 plays a crucial role in stem cells of colorectum in cooperation with Lgr5 gene. In this study, we aimed to explicate a regulatory mechanism of the KLF5 gene product from a view of three-dimensional genome structure in colorectal cancer (CRC).

Methods

In vitro engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP)-seq method was used to identify the regions that bind to the KLF5 promoter.

Results

We revealed that the KLF5 promoter region interacted with the KLF5 enhancer region as well as the transcription start site (TSS) region of the Colon Cancer Associated Transcript 1 (CCAT1) gene. Notably, the heterodeletion mutants of KLF5 enhancer impaired the cancer stem-like properties of CRC cells. The KLF5 protein participated in the

SUBMITTER: Takeda T 

PROVIDER: S-EPMC8727571 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature