Targeted Deletion of the First Intron of the Wx^b Allele via CRISPR/Cas9 Significantly Increases Grain Amylose Content in Rice.

ABSTRACT:

Background

The rice Waxy (Wx) gene plays a major role in seed amylose synthesis and consequently controls grain amylose content. Wx gene expression is highly regulated at the post-transcriptional level. In particular, the GT/TT polymorphism at the 5'splicing site of its 1st intron greatly affects this intron's splicing efficiency and defines two predominant Wx alleles, Wx^a and Wx^b. Wx^a rice often harbours intermediate to high amylose contents, whereas Wx^b rice exhibits low to intermediate amylose contents. By deleting the Wx 1st intron using CRISPR/Cas9 technology, we generate a completely novel Wx allele and further investigate how intron removal affects Wx gene expression and rice grain amylose content.

Results

CRISPR/Cas9-mediat

SUBMITTER: Liu X 

PROVIDER: S-EPMC8727654 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature