Genetic and genomic architecture in eight strains of the laboratory opossum Monodelphis domestica.

ABSTRACT: The gray short-tailed opossum (Monodelphis domestica) is an established laboratory-bred marsupial model for biomedical research. It is a critical species for comparative genomics research, providing the pivotal phylogenetic outgroup for studies of derived vs ancestral states of genomic/epigenomic characteristics for eutherian mammal lineages. To characterize the current genetic profile of this laboratory marsupial, we examined 79 individuals from eight established laboratory strains. Double digest restriction site-associated DNA sequencing and whole-genome resequencing experiments were performed to investigate the genetic architecture in these strains. A total of 66,640 high-quality single nucleotide polymorphisms (SNPs) were identified. We analyzed SNP density, average heterozygosity, nuc

SUBMITTER: Xiong X 

PROVIDER: S-EPMC8728031 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature