Use of metagenomic microbial source tracking to investigate the source of a foodborne outbreak of cryptosporidiosis.

ABSTRACT: Cryptosporidium is a protozoan parasite of global public health importance that causes gastroenteritis in a variety of vertebrate hosts, with many human outbreaks reported yearly, often from ingestion of contaminated water or food. Despite the major public health implications, little is typically known about sources of contamination of disease outbreaks caused by Cryptosporidium. Here, we study a national foodborne outbreak resulted from infection with Cryptosporidium parvum via romaine lettuce, with the main goal to trace the source of the parasite. To do so, we combined traditional outbreak investigation methods with molecular detection and characterization methods (i.e. PCR based typing, amplicon and shotgun sequencing) of romaine lettuce samples collected at the sa

SUBMITTER: Ahlinder J 

PROVIDER: S-EPMC8728467 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature