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Anti-EFG1 2'-OMethylRNA oligomer inhibits Candida albicans filamentation and attenuates the candidiasis in Galleria mellonella.

ABSTRACT: EFG1 is a central transcriptional regulator of filamentation that is an important virulence factor of Candida albicans. This study serves to assess in vivo the applicability of the anti-EFG1 2'-OMethylRNA oligomer for inhibiting C . albicans filamentation and to attenuate candidiasis, using the Galleria mellonella model. For that, larvae infected with a lethal concentration of C. albicans cells were treated with a single dose and with a double dose of the anti-EFG1 2'OMe oligomer (at 40 and 100 nM). The anti-EFG1 2'OMe oligomer toxicity and effect on larvae survival was evaluated. No evidence of anti-EFG1 2'OMe oligomer toxicity was observed and the treatment with double dose of 2'OMe oligomer empowered larvae survival over 24 h (by 90%-100%) and prolonged its efficacy until 72 h of infection (by 30%). Undoubtedly, this work validates the in vivo therapeutic potential of anti-EFG1 2'OMe oligomer for controlling C. albicans infections.

SUBMITTER: Araujo D 

PROVIDER: S-EPMC8728520 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature

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Anti-<i>EFG1</i> 2'-<i>O</i>MethylRNA oligomer inhibits <i>Candida albicans</i> filamentation and attenuates the candidiasis in <i>Galleria mellonella</i>.

Araújo Daniela D   Mil-Homens Dalila D   Henriques Mariana M   Silva Sónia S  

Molecular therapy. Nucleic acids 20211216

<i>EFG1</i> is a central transcriptional regulator of filamentation that is an important virulence factor of <i>Candida albicans</i>. This study serves to assess <i>in vivo</i> the applicability of the anti-<i>EFG1</i> 2'-<i>O</i>MethylRNA oligomer for inhibiting <i>C</i> <i>.</i> <i>albicans</i> filamentation and to attenuate candidiasis, using the <i>Galleria mellonella</i> model. For that, larvae infected with a lethal concentration of <i>C. albicans</i> cells were treated with a single dose  ...[more]

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