A single bout of cycling exercise induces nucleosome repositioning in the skeletal muscle of lean and overweight/obese individuals.

ABSTRACT:

Aim

To compare the molecular and metabolic effects of a single exercise bout in the skeletal muscle between lean and overweight/obese (Ov/Ob) individuals.

Materials and methods

Participants recruited were men, aged 19-30 years, who were either lean (body mass index [BMI] < 25, 18.5-24.1 kg/m² ; n = 15) or Ov/Ob (BMI ≥ 25, 25.5-36.9 kg/m² ; n = 15). Four hours after a high-carbohydrate breakfast (7 kcal/kg; 60% carbohydrate, 25% fat, 15% protein), participants performed a cycling exercise (50% VO₂ max, expending ~650 kcal). Muscle biopsies and peripheral blood samples were collected 30 minutes before the meal and immediately after exercise. Blood analysis, and muscle acylcarnitine profiles, transcriptomics, and nucleosome mapping by micrococca

SUBMITTER: Devarshi PP 

PROVIDER: S-EPMC8728694 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature