Reactivity and DNA Damage by Independently Generated 2'-Deoxycytidin-N4-yl Radical.

ABSTRACT: Oxidative stress produces a variety of radicals in DNA, including pyrimidine nucleobase radicals. The nitrogen-centered DNA radical 2'-deoxycytidin-N4-yl radical (dC·) plays a role in DNA damage mediated by one electron oxidants, such as HOCl and ionizing radiation. However, the reactivity of dC· is not well understood. To reduce this knowledge gap, we photochemically generated dC· from a nitrophenyl oxime nucleoside and within chemically synthesized oligonucleotides from the same precursor. dC· formation is confirmed by transient UV-absorption spectroscopy in laser flash photolysis (LFP) experiments. LFP and duplex DNA cleavage experiments indicate that dC· oxidizes dG. Transient formation of the dG radical cation (dG^+•) is observed in LFP experiments. Oxidation of the o

SUBMITTER: Peng H 

PROVIDER: S-EPMC8728764 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature