Expression of immune response genes in human corneal epithelial cells interacting with Aspergillus flavus conidia.

ABSTRACT:

Background

Aspergillus flavus, one of the causative agents of human fungal keratitis, can be phagocytosed by human corneal epithelial (HCE) cells and the conidia containing phagosomes mature into phagolysosomes. But the immunological responses of human corneal epithelial cells interacting with A. flavus are not clear. In this study, we report the expression of immune response related genes of HCE cells exposed to A. flavus spores using targeted transcriptomics.

Methods

Human corneal epithelial cell line and primary cultures were grown in a six-well plate and used for coculture experiments. Internalization of the conidia was confirmed by immunofluorescence microscopy of the colocalized endosomal markers CD71 and LAMP1. Total RNA was isolated, and the quantity and quality of t

SUBMITTER: Arunachalam D 

PROVIDER: S-EPMC8728928 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature