Single-cell analysis of human non-small cell lung cancer lesions refines tumor classification and patient stratification.

ABSTRACT: Immunotherapy is a mainstay of non-small cell lung cancer (NSCLC) management. While tumor mutational burden (TMB) correlates with response to immunotherapy, little is known about the relationship between the baseline immune response and tumor genotype. Using single-cell RNA sequencing, we profiled 361,929 cells from 35 early-stage NSCLC lesions. We identified a cellular module consisting of PDCD1⁺CXCL13⁺ activated T cells, IgG⁺ plasma cells, and SPP1⁺ macrophages, referred to as the lung cancer activation module (LCAM^hi). We confirmed LCAM^hi enrichment in multiple NSCLC cohorts, and paired CITE-seq established an antibody panel to identify LCAM^hi lesions. LCAM presence was found to be independent of overall immune

SUBMITTER: Leader AM 

PROVIDER: S-EPMC8728963 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature