Characterization of the O-Glycoproteome of Porphyromonas gingivalis.

ABSTRACT: Porphyromonas gingivalis is an important human pathogen and also a model organism for the Bacteroidetes phylum. O-glycosylation has been reported in this phylum with findings that include the O-glycosylation motif, the structure of the O-glycans in a few species, and an extensive O-glycoproteome analysis in Tannerella forsythia. However, O-glycosylation has not yet been confirmed in P. gingivalis. We therefore used glycoproteomics approaches including partial deglycosylation with trifluoromethanesulfonic acid as well as both HILIC and FAIMS based glycopeptide enrichment strategies leading to the identification of 257 putative glycosylation sites in 145 glycoproteins. The sequence of the major O-glycan was elucidated to be HexNAc-HexNAc(P-Gro-[Ac]_0-2)-dHex-Hex-HexA-Hex(dHex). Wes

SUBMITTER: Veith PD 

PROVIDER: S-EPMC8729774 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature