Scalable Isolation and Purification of Extracellular Vesicles from Escherichia coli and Other Bacteria.

ABSTRACT: Diverse bacterial species secrete ~20-300 nm extracellular vesicles (EVs), comprised of lipids, proteins, nucleic acids, glycans, and other molecules derived from the parental cells. EVs function as intra- and inter-species communication vectors while also contributing to the interaction between bacteria and host organisms in the context of infection and colonization. Given the multitude of functions attributed to EVs in health and disease, there is a growing interest in isolating EVs for in vitro and in vivo studies. It was hypothesized that the separation of EVs based on physical properties, namely size, would facilitate the isolation of vesicles from diverse bacterial cultures. The isolation workflow consists of centrifugation, filtration, ultrafiltration, and size-exclusion chromatogra

SUBMITTER: Watson DC 

PROVIDER: S-EPMC8729794 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature