Methylation of SDC2/TFPI2 and Its Diagnostic Value in Colorectal Tumorous Lesions.

ABSTRACT: Background: SDC2 methylation is a feasible biomarker for colorectal cancer detection. Its specificity for colorectal cancer is higher than 90%, but the sensitivity is normally lower than 90%. This study aims to improve the sensitivity of SDC2 detection through finding a high positive target from the false-negative samples of SDC2 detection based on analysis of the bowel subsite difference in methylation. Methods: Hypermethylated TFPI2 was identified in SDC2 hypomethylated colorectal cancer samples retrieved from TCGA database with the methylation level lower than 0.2. The methylation-specific PCR assay was developed and then evaluated using tissue samples (184 cancer and 54 healthy control samples) and stool samples (289 cancer, 190 adenoma,

SUBMITTER: Zhang L 

PROVIDER: S-EPMC8729808 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature