Internal conversion of the anionic GFP chromophore: in and out of the I-twisted S₁/S₀ conical intersection seam.

ABSTRACT: The functional diversity of the green fluorescent protein (GFP) family is intimately connected to the interplay between competing photo-induced transformations of the chromophore motif, anionic p-hydroxybenzylidene-2,3-dimethylimidazolinone (HBDI^-). Its ability to undergo Z/E-isomerization is of particular importance for super-resolution microscopy and emerging opportunities in optogenetics. Yet, key dynamical features of the underlying internal conversion process in the native HBDI^- chromophore remain largely elusive. We investigate the intrinsic excited-state behavior of isolated HBDI^- to resolve competing decay pathways and map out the factors governing efficiency and the stereochemical outcome of photoisomerization. Based on non-adi

SUBMITTER: List NH 

PROVIDER: S-EPMC8729814 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature