Comprehensive Search for Novel Circulating miRNAs and Axon Guidance Pathway Proteins Associated with Risk of ESKD in Diabetes.

ABSTRACT:

Background

Mechanisms underlying the pro gression of diabetic kidney disease to ESKD are not fully understood.

Methods

We performed global microRNA (miRNA) analysis on plasma from two cohorts consisting of 375 individuals with type 1 and type 2 diabetes with late diabetic kidney disease, and targeted proteomics analysis on plasma from four cohorts consisting of 746 individuals with late and early diabetic kidney disease. We examined structural lesions in kidney biopsy specimens from the 105 individuals with early diabetic kidney disease. Human umbilical vein endothelial cells were used to assess the effects of miRNA mimics or inhibitors on regulation of candidate proteins.

Results

In the late diabetic kidney disease cohorts, we identified 17 circulating miRNAs, repres

SUBMITTER: Satake E 

PROVIDER: S-EPMC8729832 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature