Human Kidney Spheroids and Monolayers Provide Insights into SARS-CoV-2 Renal Interactions.

ABSTRACT:

Background

Although coronavirus disease 2019 (COVID-19) causes significan t morbidity, mainly from pulmonary involvement, extrapulmonary symptoms are also major componen ts of the disease. Kidney disease, usually presenting as AKI, is particularly severe among patients with COVID-19. It is unknown, however, whether such injury results from direct kidney infection with COVID-19's causative virus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), or from indirect mechanisms.

Methods

Using ex vivo cell models, we sought to analyze SARS-CoV-2 interactions with kidney tubular cells and assess direct tubular injury. These models comprised primary human kidney epithelial cells (derived from nephrectomies) and grown as either proliferating monolayers or quiescent

SUBMITTER: Omer D 

PROVIDER: S-EPMC8729846 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature