Slit2-Robo Signaling Promotes Glomerular Vascularization and Nephron Development.

ABSTRACT:

Background

Kidney function requires continuous blood filtration by glomerular capillaries. Disruption of glomerular vascular development or maintenance contributes to the pathogenesis of kidney diseases, but the signaling events regulating renal endothelium development remain incompletely understood. Here, we discovered a novel role of Slit2-Robo signaling in glomerular vascularization. Slit2 is a secreted polypeptide that binds to transmembrane Robo receptors and regulates axon guidance as well as ureteric bud branching and angiogenesis.

Methods

We performed Slit2-alkaline phosphatase binding to kidney cryosections from mice with or without tamoxifen-inducible Slit2 or Robo1 and -2 deletions, and we characterized the phenotypes using immunohistochemistry, elec

SUBMITTER: Li J 

PROVIDER: S-EPMC8729857 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature