MiR-214-5p Regulating Differentiation of Intramuscular Preadipocytes in Goats via Targeting KLF12.

ABSTRACT: Intramuscular fat (i.m.) is an adipose tissue that is deposited between muscle bundles. An important type of post-transcriptional regulatory factor, miRNAs, has been observed as an important regulator that can regulate gene expression and cell differentiation through specific binding with target genes, which is the pivotal way determining intramuscular fat deposition. Thus, this study intends to use RT-PCR, cell culture, liposome transfection, real-time fluorescent quantitative PCR (qPCR), dual luciferase reporter systems, and other biological methods clarifying the possible mechanisms on goat intramuscular preadipocyte differentiation that is regulated by miR-214-5p. Ultimately, our results showed that the expression level of miR-214-5p peaked at 48 h after the goat intramuscular preadipo

SUBMITTER: Du Y 

PROVIDER: S-EPMC8730364 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature