Rapid, Affordable, and Scalable SARS-CoV-2 Detection From Saliva.

ABSTRACT: Here we present an inexpensive, rapid, and robust reverse-transcription loop-mediated isothermal amplification (RT-LAMP)-based severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection method that is easily scalable, enabling point-of-care facilities and clinical labs to determine results from patients' saliva directly in 30 minutes for less than $2 per reaction. The method uses a novel combination of widely available reagents that can be prepared in bulk, plated, and frozen and remain stable until samples are received. This innovation dramatically reduces preparation time, enabling high-throughput automation and testing with time to results (including setup) in less than 1 hour for 96 patient samples simultaneously when using a 384-well format. By using a dual reporter (pheno

SUBMITTER: Hayden A 

PROVIDER: S-EPMC8730515 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature