The Protective Role of 1,8-Dihydroxynaphthalene-Melanin on Conidia of the Opportunistic Human Pathogen Aspergillus fumigatus Revisited: No Role in Protection against Hydrogen Peroxide and Superoxides.

ABSTRACT: Previously, 1,8-dihydroxynaphthalene (DHN)-melanin was described to protect Aspergillus fumigatus against hydrogen peroxide (H₂O₂), thereby protecting this opportunistic human pathogen from reactive oxygen species generated by the immune system. This was based on the finding that the ATCC 46645 mutant with mutations in the pksP gene of the DHN-melanin synthesis pathway showed increased sensitivity to reactive oxygen species compared to the wild type. Here, it is shown that deletion of the pksP gene in A. fumigatus strain CEA10 did not affect sensitivity for H₂O₂ and superoxide in a plate stress assay. In addition, direct exposure of the dormant white conidia of the pksP deletion strains to H₂O₂ did not r

SUBMITTER: Keizer EM 

PROVIDER: S-EPMC8730813 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature