CRISPRi links COVID-19 GWAS loci to LZTFL1 and RAVER1.

ABSTRACT:

Background

To identify host genetic variants (SNPs) associated with COVID-19 disease severity, a number of genome-wide association studies (GWAS) have been conducted. Since most of the identified variants are located at non-coding regions, such variants are presumed to affect the expression of neighbouring genes, thereby influencing COVID-19 disease severity. However, it remains largely unknown which genes are influenced by such COVID-19 GWAS loci.

Methods

CRISPRi (interference)-mediated gene expression analysis was performed to identify genes functionally regulated by COVID-19 GWAS loci by targeting regions near the loci (SNPs) in lung epithelial cell lines. The expression of CRISPRi-identified genes was investigated using COVID-19-contracted human and monkey lung single-nu

SUBMITTER: Fink-Baldauf IM 

PROVIDER: S-EPMC8731227 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature