Integrating cultivation and metagenomics for a multi-kingdom view of skin microbiome diversity and functions.

ABSTRACT: Human skin functions as a physical barrier to foreign pathogen invasion and houses numerous commensals. Shifts in the human skin microbiome have been associated with conditions ranging from acne to atopic dermatitis. Previous metagenomic investigations into the role of the skin microbiome in health or disease have found that much of the sequenced data do not match reference genomes, making it difficult to interpret metagenomic datasets. We combined bacterial cultivation and metagenomic sequencing to assemble the Skin Microbial Genome Collection (SMGC), which comprises 622 prokaryotic species derived from 7,535 metagenome-assembled genomes and 251 isolate genomes. The metagenomic datasets that we generated were combined with publicly available skin metagenomic datasets to identify members a

SUBMITTER: Saheb Kashaf S 

PROVIDER: S-EPMC8732310 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature