Establishment of Down's syndrome periodontal ligament cells by transfection with SV40T-Ag and hTERT.

ABSTRACT: Down's syndrome is one of the most common human congenital genetic diseases and affected patients have increased risk of periodontal disease. To examine involvement of the disease with periodontal disease development, we established immortalized periodontal ligament cells obtained from a Down's syndrome patient by use of SV40T-Ag and hTERT gene transfection. Expressions of SV40T-Ag and hTERT were observed in periodontal ligament cell-derived immortalized cells established from healthy (STPDL) and Down's syndrome patient (STPDLDS) samples. Primary cultured periodontal ligament cells obtained from a healthy subject (pPDL) had a limited number of population doublings (< 40), while STPDL and STPDLDS cells continued to grow with more than 80 population doublings. Primary cultured periodontal li

SUBMITTER: Asakawa T 

PROVIDER: S-EPMC8732922 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature