High-Throughput Strategy for Profiling Sequential Section With Multiplex Staining of Mouse Brain.

ABSTRACT: The brain modulates specific functions in its various regions. Understanding the organization of different cells in the whole brain is crucial for investigating brain functions. Previous studies have focused on several regions and have had difficulty analyzing serial tissue samples. In this study, we introduced a pipeline to acquire anatomical and histological information quickly and efficiently from serial sections. First, we developed a serial brain-slice-staining method to stain serial sections and obtained more than 98.5% of slices with high integrity. Subsequently, using the self-developed analysis software, we registered and quantified the signals of imaged sections to the Allen Mouse Brain Common Coordinate Framework, which is compatible with multimodal images and slant section plan

SUBMITTER: Chen S 

PROVIDER: S-EPMC8732995 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature