Evaluation of CRISPR gene-editing tools in zebrafish.

ABSTRACT:

Background

Zebrafish have practical features that make them a useful model for higher-throughput tests of gene function using CRISPR/Cas9 editing to create 'knockout' models. In particular, the use of G₀ mosaic mutants has potential to increase throughput of functional studies significantly but may suffer from transient effects of introducing Cas9 via microinjection. Further, a large number of computational and empirical tools exist to design CRISPR assays but often produce varied predictions across methods leaving uncertainty in choosing an optimal approach for zebrafish studies.

Methods

To systematically assess accuracy of tool predictions of on- and off-target gene editing, we subjected zebrafish embryos to CRISPR/Cas9 with 50 different guide RNAs (gRNAs) targe

SUBMITTER: Uribe-Salazar JM 

PROVIDER: S-EPMC8734261 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature