ZNF750 exerted its Antitumor Action in Oral Squamous Cell Carcinoma by regulating E2F2.

ABSTRACT: Cell cycle activator E2F transcription factor 2 (E2F2) play a key role in tumor development and metastasis. Previous RNA sequence analysis (GSE134835) revealed E2F2 was significantly reduced by Zinc-finger protein 750 (ZNF750) in oral squamous cell carcinoma (OSCC). This study was aimed to determine the involvement of E2F2 in antitumor action of ZNF750. The nude mouse xenograft model was established by subcutaneously injection of stable cell line CAL-27^oeZNF750 or CAL-27^shZNF750. Xenograft tumor volume and tumor weight was measured. The expression of E2F2, transcriptional repressors such as enhancer of zeste 2 (Ezh2), PHD finger protein 19 (PHF19), and the genes related to cell proliferation or metastasis was studied in vivo or in vitro. Luciferase assay

SUBMITTER: Yang HL 

PROVIDER: S-EPMC8734408 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature