Glycolysis/gluconeogenesis specialization in microbes is driven by biochemical constraints of flux sensing.

ABSTRACT: Central carbon metabolism is highly conserved across microbial species, but can catalyze very different pathways depending on the organism and their ecological niche. Here, we study the dynamic reorganization of central metabolism after switches between the two major opposing pathway configurations of central carbon metabolism, glycolysis, and gluconeogenesis in Escherichia coli, Pseudomonas aeruginosa, and Pseudomonas putida. We combined growth dynamics and dynamic changes in intracellular metabolite levels with a coarse-grained model that integrates fluxes, regulation, protein synthesis, and growth and uncovered fundamental limitations of the regulatory network: After nutrient shifts, metabolite concentrations collapse to their equilibrium, rendering the cell unable to sense which direct

SUBMITTER: Schink SJ 

PROVIDER: S-EPMC8738977 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature