Chromogenic Escherichia coli reporter strain for screening DNA damaging agents.

ABSTRACT: The presence of pollutants in soil and water has given rise to diverse analytical and biological approaches to detect and measure contaminants in the environment. Using bacterial cells as reporter strains represents an advantage for detecting pollutants present in soil or water samples. Here, an Escherichia coli reporter strain expressing a chromoprotein capable of interacting with soil or water samples and responding to DNA damaging compounds is validated. The reporter strain generates a qualitative signal and is based on the expression of the coral chromoprotein AmilCP under the control of the recA promoter. This strain can be used simply by applying soil or water samples directly and rendering activation upon DNA damage. This reporter strain responds to agents that damage DNA (with an a

SUBMITTER: Mora-Garduno JD 

PROVIDER: S-EPMC8739417 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature