Rapid extraction from and direct identification in clinical samples of methicillin-resistant staphylococci using the PCR.

ABSTRACT: Methicillin-resistant staphylococci (MRS) are one of the most common causes of nosocomial infections and bacteremia. Standard bacterial identification and susceptibility testing frequently require as long as 72 h to report results, and there may be difficulty in rapidly and accurately identifying methicillin resistance. The use of the PCR is a rapid and simple process for the amplification of target DNA sequences, which can be used to identify and test bacteria for antimicrobial resistance. However, many sample preparation methods are unsuitable for PCR utilization in the clinical laboratory because they either are not cost-effective, take too long to perform, or do not provide a satisfactory DNA template for PCR. Our goal was to provide same-day results to facilitate rapid diagnosis and t

SUBMITTER: Jaffe RI 

PROVIDER: S-EPMC87395 | biostudies-literature | 2000 Sep

REPOSITORIES: biostudies-literature