Automated 3D Sampling and Imaging of Uneven Sample Surfaces with LA-REIMS.

ABSTRACT: The analysis of samples with large height variations remains a challenge for mass spectrometry imaging (MSI), despite many technological advantages. Ambient sampling and ionization MS techniques allow for the molecular analysis of sample surfaces with height variations, but most techniques lack MSI capabilities. We developed a 3D MS scanner for the automated sampling and imaging of a 3D surface with laser-assisted rapid evaporative ionization mass spectrometry (LA-REIMS). The sample is moved automatically with a constant distance between the laser probe and sample surface in the 3D MS Scanner. The topography of the surface was scanned with a laser point distance sensor to define the MS measurement points. MS acquisition was performed with LA-REIMS using a surgical CO₂ laser coup

SUBMITTER: Nauta SP 

PROVIDER: S-EPMC8739836 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature