Transient recombinant expression of highly immunogenic CagA, VacA and NapA in Nicotiana benthamiana.

ABSTRACT: Interest in the plant-based transient production of recombinant immunogenic antigens has tremendously progressed because plants are cost-effective, easily selectable, free of mammalian contamination, and support complex post-translational modifications. Nicotiana benthamiana is a convenient system for transient expression of recombinant antigens. The present study documented a platform for rapid production of Helicobacter pylori CagA, VacA and NapA antigens three days (first harvest, FH) and six days (second harvest, SH) after agro-infiltration using a syringe. In this study, CagA, VacA and NapA antigen genes from Helicobacter pylori were cloned into the binary vector pBI121 and transformed into Nicotiana benthamiana by the Agrobacterium<

SUBMITTER: Barzigar R 

PROVIDER: S-EPMC8739878 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature