Probing Differences in Gene Essentiality Between the Human and Animal Adapted Lineages of the <i>Mycobacterium tuberculosis</i> Complex Using TnSeq.

ABSTRACT: Members of the Mycobacterium tuberculosis complex (MTBC) show distinct host adaptations, preferences and phenotypes despite being >99% identical at the nucleic acid level. Previous studies have explored gene expression changes between the members, however few studies have probed differences in gene essentiality. To better understand the functional impacts of the nucleic acid differences between Mycobacterium bovis and Mycobacterium tuberculosis, we used the Mycomar T7 phagemid delivery system to generate whole genome transposon libraries in laboratory strains of both species and compared the essentiality status of genes during growth under identical in vitro conditions. Libraries contained insertions in 54% of possible TA sites in M. bovis and 40% of thos

SUBMITTER: Gibson AJ 

PROVIDER: S-EPMC8739905 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature