MicroRNA-210 regulates the metabolic and inflammatory status of primary human astrocytes.

ABSTRACT:

Background

Astrocytes are the most numerous glial cell type with important roles in maintaining homeostasis and responding to diseases in the brain. Astrocyte function is subject to modulation by microRNAs (miRs), which are short nucleotide strands that regulate protein expression in a post-transcriptional manner. Understanding the miR expression profile of astrocytes in disease settings provides insight into the cellular stresses present in the microenvironment and may uncover pathways of therapeutic interest.

Methods

Laser-capture microdissection was used to isolate human astrocytes surrounding stroke lesions and those from neurological control tissue. Astrocytic miR expression profiles were examined using quantitative reverse transcription polymerase chain reaction (RT-qP

SUBMITTER: Kieran NW 

PROVIDER: S-EPMC8740343 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature