Light-induced production of isobutanol and 3-methyl-1-butanol by metabolically engineered cyanobacteria.

ABSTRACT:

Background

Cyanobacteria are engineered via heterologous biosynthetic pathways to produce value-added chemicals via photosynthesis. Various chemicals have been successfully produced in engineered cyanobacteria. Chemical inducer-dependent promoters are used to induce the expression of target biosynthetic pathway genes. A chemical inducer is not ideal for large-scale reactions owing to its high cost; therefore, it is important to develop scaling-up methods to avoid their use. In this study, we designed a green light-inducible alcohol production system using the CcaS/CcaR green light gene expression system in the cyanobacterium Synechocystis sp. PCC 6803 (PCC 6803).

Results

To establish the green light-inducible production of isobutanol and 3-methyl-1-butanol (3MB) in PCC 6803,

SUBMITTER: Kobayashi S 

PROVIDER: S-EPMC8740407 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature