Argonaute-2 protects the neurovascular unit from damage caused by systemic inflammation.

ABSTRACT:

Background

The brain vasculature plays a pivotal role in the inflammatory process by modulating the interaction between blood cells and the neurovascular unit. Argonaute-2 (Ago2) has been suggested as essential for endothelial survival but its role in the brain vasculature or in the endothelial-glial crosstalk has not been addressed. Thus, our aim was to clarify the significance of Ago2 in the inflammatory responses elicited by these cell types.

Methods

Mouse primary cultures of brain endothelial cells, astrocytes and microglia were used to evaluate cellular responses to the modulation of Ago2. Exposure of microglia to endothelial cell-conditioned media was used to assess the potential for in vivo studies. Adult mice were injected intraperitoneally with lipopolysaccharide (L

SUBMITTER: Machado-Pereira M 

PROVIDER: S-EPMC8740421 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature