RNA biomarkers from proximal liquid biopsy for diagnosis of ovarian cancer.

ABSTRACT:

Background

Most ovarian cancer patients are diagnosed at an advanced stage and have a high mortality rate. Current screening strategies fail to improve prognosis because markers that are sensitive for early stage disease are lacking. This medical need justifies the search for novel approaches using utero-tubal lavage as a proximal liquid biopsy.

Methods

In this study, we explore the extracellular transcriptome of utero-tubal lavage fluid obtained from 26 ovarian cancer patients and 48 controls using messenger RNA (mRNA) capture and small RNA sequencing.

Results

We observed an enrichment of ovarian and fallopian tube specific messenger RNAs in utero-tubal lavage fluid compared to other human biofluids. Over 300 mRNAs and 41 miRNAs were upregulated in ovarian cancer sam

SUBMITTER: Hulstaert E 

PROVIDER: S-EPMC8740458 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature