Ultrasensitive dynamic light scattering immunosensing platform for NT-proBNP detection using boronate affinity amplification.

ABSTRACT: Herein, we reported a new dynamic light scattering (DLS) immunosensing technology for the rapid and sensitive detection of glycoprotein N-terminal pro-brain natriuretic peptide (NT-proBNP). In this design, the boronate affinity recognition based on the interaction of boronic acid ligands and cis-diols was introduced to amplify the nanoparticle aggregation to enable highly sensitive DLS transduction, thereby lowering the limit of detection (LOD) of the methodology. After covalently coupling with antibodies, magnetic nanoparticles (MNPs) were employed as the nanoprobes to selectively capture trace amount of NT-proBNP from complex samples and facilitate DLS signal transduction. Meanwhile, silica nanoparticles modified with phenylboronic acid (SiO₂@PBA) were designed as the crosslin

SUBMITTER: Hu J 

PROVIDER: S-EPMC8740487 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature