Integrative profiling of Epstein-Barr virus transcriptome using a multiplatform approach.

ABSTRACT:

Background

Epstein-Barr virus (EBV) is an important human pathogenic gammaherpesvirus with carcinogenic potential. The EBV transcriptome has previously been analyzed using both Illumina-based short read-sequencing and Pacific Biosciences RS II-based long-read sequencing technologies. Since the various sequencing methods have distinct strengths and limitations, the use of multiplatform approaches have proven to be valuable. The aim of this study is to provide a more complete picture on the transcriptomic architecture of EBV.

Methods

In this work, we apply the Oxford Nanopore Technologies MinION (long-read sequencing) platform for the generation of novel transcriptomic data, and integrate these with other's data generated by another LRS approach, Pacific BioSciences RSII seque

SUBMITTER: Fulop A 

PROVIDER: S-EPMC8740505 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature