PCR for diagnosis of paracoccidioidomycosis.

ABSTRACT: A PCR assay based on oligonucleotide primers derived from the sequence of the gene coding for the 43,000-Da (gp43) antigen was developed to detect Paracoccidioides brasiliensis DNA in sputa. In the standardized conditions, it could detect 10 cells/ml of sputum, providing sufficient accuracy to be useful for diagnosis of paracoccidioidomycosis.

SUBMITTER: Gomes GM 

PROVIDER: S-EPMC87412 | biostudies-literature | 2000 Sep

REPOSITORIES: biostudies-literature