Medium-chain fatty acids enhance expression and histone acetylation of genes related to lipid metabolism in insulin-resistant adipocytes.

ABSTRACT:

Background

The expressions of genes related to lipid metabolism are decreased in adipocytes with insulin resistance. In this study, we examined the effects of fatty acids on the reduced expressions and histone acetylation of lipid metabolism-related genes in 3T3-L1 adipocytes treated with insulin resistance induced by tumor necrosis factor (TNF)-α.

Methods

Short-, medium-, and long-chain fatty acid were co-administered with TNF-α in 3T3-L1 adipocytes. Then, mRNA expressions and histone acetylation of genes involved in lipid metabolism were determined using mRNA microarrays, qRT-PCR, and chromatin immunoprecipitation assays.

Results

We found in microarray and subsequent qRT-PCR analyses that the expression levels of several lipid metabolism-related genes, including

SUBMITTER: Kawamura M 

PROVIDER: S-EPMC8741418 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature