SATB2 preserves colon stem cell identity and mediates ileum-colon conversion via enhancer remodeling.

ABSTRACT: Adult stem cells maintain regenerative tissue structure and function by producing tissue-specific progeny, but the factors that preserve their tissue identities are not well understood. The small and large intestines differ markedly in cell composition and function, reflecting their distinct stem cell populations. Here we show that SATB2, a colon-restricted chromatin factor, singularly preserves LGR5⁺ adult colonic stem cell and epithelial identity in mice and humans. Satb2 loss in adult mice leads to stable conversion of colonic stem cells into small intestine ileal-like stem cells and replacement of the colonic mucosa with one that resembles the ileum. Conversely, SATB2 confers colonic properties on the mouse ileum. Human colonic organoids also adopt ileal characteristics upon

SUBMITTER: Gu W 

PROVIDER: S-EPMC8741647 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature