Single cell analysis reveals inhibition of angiogenesis attenuates the progression of heterotopic ossification in Mkx^-/- mice.

ABSTRACT: Tendon heterotopic ossification (HO) is characterized by bone formation inside tendon tissue, which severely debilitates people in their daily life. Current therapies fail to promote functional tissue repair largely due to our limited understanding of HO pathogenesis. Here, we investigate the pathological mechanism and propose a potential treatment method for HO. Immunofluorescence assays showed that the Mohawk (MKX) expression level was decreased in human tendon HO tissue, coinciding with spontaneous HO and the upregulated expression of osteochondrogenic and angiogenic genes in the tendons of Mkx^-/- mice. Single-cell RNA sequencing analyses of wild-type and Mkx^-/- tendons identified three cell types and revealed the excessive activation of osteochondrogenic genes dur

SUBMITTER: Lin J 

PROVIDER: S-EPMC8741758 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature